Inhibition of c-MYC-miRNA 19 Pathway Sensitized CML K562 Cells to Etoposide via NHE1 Upregulation

Figure 1

NHE1 affected the sensitivity of different cell lines to apoptosis. (a, b) MTT analysis of cells that were exposed to Cariporide (Cari) with or without Etoposide (Eto). OD values of untreated control cells were set as 100%. All other values refer to the untreated control. (c) Western blotting indicating the efficiency of pECE-NHE1 and two shRNA (sh1 and sh2) to NHE1 was carried out in MCF-7 and MDA-MB-231 cells. (d, e) Apoptosis of MCF-7 and MDA-MB-231cells transfected with indicated plasmids or treated with Cariporide was detected. Cells were transfected and then treated with Etoposide for 24 h. (f) Apoptosis of different hematopoietic cell lines exposed to Cariporide with or without Etoposide was shown. (g) Western blotting indicating the efficiency of two shRNA (sh1 and sh2) to NHE1 was carried out in K562 cells. (h) K562 cells were transfected with indicated shNHE1 plasmid or treated with Cariporide and then exposed to Etoposide. The apoptosis was shown. (i, j) K562 cells were transfected with indicated shNHE1 plasmid or treated with Cariporide and then exposed to Imatinib. The apoptosis and viability curves were shown. Data was shown as of triplicate assays. . . . .

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