Inhibition of c-MYC-miRNA 19 Pathway Sensitized CML K562 Cells to Etoposide via NHE1 Upregulation

Figure 2

Etoposide altered NHE1 expression and pHi value among hematopoietic cell lines. (a) The mRNA expression level of NHE1 in HL-60, Jurkat, and K562 cells treated with Etoposide was detected. (b) The mRNA expression level of NHE1 in HL-60, Jurkat, and K562 cells treated with Irradiate was detected. (c, d) The protein expression level of NHE1 in HL-60 and Jurkat cells treated with Etoposide or in K562 cells treated with Imatinib was detected. Densitometric value was normalized by β-actin and showed the fold of treatment versus control. (e) The pHi in HL-60, Jurkat, and K562 cells treated with Etoposide was detected. (f) The pHi in HL-60, Jurkat, and K562 cells treated with Irradiate was detected. (g, h) The pHi in K562 cells with indicated treatments was detected. Data was shown as of triplicate assays. . . . .

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