Inhibition of c-MYC-miRNA 19 Pathway Sensitized CML K562 Cells to Etoposide via NHE1 Upregulation

<div>Characterization of NHE1 promoter activity responded to Etoposide. (a) SLC9A1 promoter activity responded to serum deprivation. (b) Luciferase activity in K562 cells transfected with PGL3-1360 and incubated with Etoposide at different final concentrations was detected. (c) Response of PGL3-1360 and its progressive deletion regions in K562 cells to Etoposide was clarified by relative luciferase activity. (d) A schematic diagram of the proximal SLC9A1 promoter indicating 2 potential OCT1 transcription factor binding sites. (e) Biotinylated oligo pull-down assay was performed to show the binding between OCT1 and target sequence. 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Oxidative Medicine and Cellular Longevity

fig3

Figure 3

Figure 3: Inhibition of c-MYC-miRNA 19 Pathway Sensitized CML K562 Cells to Etoposide via NHE1 Upregulation 