Inhibition of c-MYC-miRNA 19 Pathway Sensitized CML K562 Cells to Etoposide via NHE1 Upregulation

Figure 5

miR-19 and c-MYC affected apoptosis of K562 cells treated with Etoposide both in vitro and in vivo. (a, b) Inhibition of miR-19 or c-MYC showed synergistic effect with Etoposide treatment on K562 cell apoptosis. (c, d) Inhibition of miR-19 or c-MYC further inhibited tumorigenicity potential of K562 cells treated with Etoposide in vivo. Representative photographs of tumors taken at the time of killing were shown. The tumor volume for each mouse was determined (in cubic millimeter) by measuring in two directions and calculated as . The tumor growth curve was accordingly shown. (e, f) TCGA data showed the negative correlation between miR-19 expression level and overall survival of leukemia patients. The patients’ overall survival curve was accordingly shown. Data was shown as of triplicate assays. . . . .

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