DL-3-N-Butylphthalide Promotes Cartilage Extracellular Matrix Synthesis and Inhibits Osteoarthritis Development by Regulating FoxO3a

Figure 4

NBP inhibited apoptosis and oxidative stress of human OA chondrocytes. (a, c) Apoptosis was analyzed by flow cytometry and apoptosis rates are expressed in the respective group. (b, d) Apoptotic assay by TUNEL staining was observed under a microscope, and TUNEL-positive cells were calculated (, scale bar, 100 μm). (e, f) The mRNA levels of Bcl-2 and Bax were measured by qRT-PCR. (g) The protein expressions of CAT, SOD2, Bcl-2, Bax, and Caspase 3 were determined after treatment with NBP (0-10 μM) for 24 h by Western blot analysis. (h, i) The expression levels of SOD and GSH in chondrocytes treated with NBP (0-10 μM) for 24 h. (j, k) The mRNA levels of SOD2 and CAT were measured by qRT-PCR. All data are expressed as the (SD). .

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