DL-3-N-Butylphthalide Promotes Cartilage Extracellular Matrix Synthesis and Inhibits Osteoarthritis Development by Regulating FoxO3a

<div>NBP increases ECM anabolism in human OA chondrocytes by regulating FoxO3a. (a, b) The protein and mRNA levels of FoxO3a were evaluated by Western blot and qRT-PCR after treated with and/or Lv-FoxO3a for 24 h. (c) Proteins expressions of ACAN and COL2A1 were detected by Western blot. (d) Immunofluorescence staining for COLII (scale bar, 50 <i>μ</i>m). (e–h) The mRNA levels of COL2A1, ACAN, SOX9, and PRG4 were determined by qRT-PCR. 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Oxidative Medicine and Cellular Longevity

fig7

Figure 7

Figure 7: DL-3-N-Butylphthalide Promotes Cartilage Extracellular Matrix Synthesis and Inhibits Osteoarthritis Development by Regulating FoxO3a 