Research Article
NFKB1 Gene Mutant Was Associated with Prognosis of Coronary Artery Disease and Exacerbated Endothelial Mitochondrial Fission and Dysfunction
Figure 2
HUVECs with the NFKB1 gene DD mutant were more vulnerable to high-glucose/palmitate-induced apoptosis. (a) FACS analysis of Annexin V-FITC/PI-labeled HUVECs was performed to quantify apoptosis. Cells in the lower right quadrant indicated Annexin-positive/PI-negative, early apoptotic cells. (b) The percentage of cells undergoing early apoptosis in comparison with the respective control after 3 h high-glucose/palmitate treatment (). (c) Representative western blot bands of apoptotic-related proteins after treatment with high glucose/palmitate in II genotype and DD mutant HUVECs. (d) The bar graphs showed the relative levels of cleaved-capase3, p65, phospho-p65, and p50, respectively (). Values are . , , and .
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