Downregulation of HULC Induces Ferroptosis in Hepatocellular Carcinoma via Targeting of the miR-3200-5p/ATF4 Axis

Figure 2

Downregulation of HULC induces ferroptosis. (a) Cell apoptosis was evaluated following transfection of si-HULC and si-NC by flow cytometry and western blotting analysis (c, d). (b) MTT assay was used to observe the activity of HCC cells treated with si-HULC in the presence of Ferrostatin-1. (e–g) MDA, GSH, and ROS were used for examining oxidative stress within cells after HULC knockdown. Three independent experiments and sample size (), compared with the control group; ; ; .

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