Downregulation of HULC Induces Ferroptosis in Hepatocellular Carcinoma via Targeting of the miR-3200-5p/ATF4 Axis

Figure 3

HULC targets miR-3200-5p and acts as a ceRNA. (a, b) Results of high-throughput sequencing identified a total of 9 significant differential genes. (c) Results of GO analysis revealed that target genes were related to selenocysteine lyase activity, peroxisomal mechanism targeting signal-1 binding, and tumor necrosis factor receptor superfamily binding. (d) KEGG pathway analysis demonstrated that selenium compound metabolism and neuroactive ligand receptor interaction along with the Wnt and JAK-STAT signaling pathways were the most abundant. (e) Results from the target prediction indicated that HULC might interact with miR-3200-5p. (f) qRT-PCR was performed to assess gene changes in miR-3200-5p after knockdown of HULC. (g) When the mimic miR-3200-5p was added, a decrease in HULC expression was observed in BEL-7402 and SMMC-7721 cells. Three independent experiments and sample size (), compared with the control group; ; .

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