Downregulation of HULC Induces Ferroptosis in Hepatocellular Carcinoma via Targeting of the miR-3200-5p/ATF4 Axis

Figure 5

lncRNA HULC mediates ferroptosis through miR-3200-5p. (a, b) BEL-7402 and SMMC-7721 cells were cotransfected with si-HULC and a miR-3200-5p mimic/inhibitor; MTT levels were used to assess cell viability. (c, d) si-HULC and inhibitor miR-3200-5p were used to detect the production of Fe²⁺ and ROS, reflecting the ferroptosis of cells. (e, f) Western blot was used to detect the expression of ferroptosis-related protein GPX4 in hepatocellular carcinoma cells. Three independent experiments and sample size (), compared with the control group; ; ; .

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