Downregulation of HULC Induces Ferroptosis in Hepatocellular Carcinoma via Targeting of the miR-3200-5p/ATF4 Axis

Figure 6

miR-3200-5p targeted ATF4. (a) The expression of ATF4 in HCC () and normal liver () tissues was detected with the use of immunohistochemistry. (b) The results of the double luciferase reporter gene showed that the gene ATF4 was associated with miR-3200-5p. (c, d) qRT-PCR was performed to detect the expression of ATF4 mRNA in HCC cells transfected with miR-3200-5p mimic/inhibitor. (e–h) Western blot was performed to detect ATF4 expression following the addition of the mimic miR-3200-5p, inhibitor miR-3200-5p, inhibitor miR-3200-5p+si-NC, or inhibitor miR-3200-5p+si-ATF4 in BEL-7402 and SMMC-7721 cells. Three independent experiments and sample size (), compared with the control group; ; ; .

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