Downregulation of HULC Induces Ferroptosis in Hepatocellular Carcinoma via Targeting of the miR-3200-5p/ATF4 Axis

Figure 7

miR-3200-5p regulates ferroptosis via targeting ATF4. (a) Results of the MTT assay revealed that ATF4 siRNA transfection restored the capacity for promotion of proliferation as produced by the miR-3200-5p inhibitor. (b) Iron detection assay was used to detect ferroptosis after adding inhibitor miR-3200-5p and si-ATF4 into HCC cells. (c) Western blot was used to detect the effects of inhibitor miR-3200-5p and si-ATF4 on the levels of ferroptosis-related protein GPX4 in HCC cells. Three independent experiments and sample size (), compared with the control group; ; ; .

(a)

(b)

(c)