Research Article
IL-22 Protects against Biliary Ischemia-Reperfusion Injury after Liver Transplantation via Activating STAT3 and Reducing Apoptosis and Oxidative Stress Levels In Vitro and In Vivo
Figure 1
IL-22 receptor is expressed in bile duct tissue or cells, and IL-22 reduces CoCl2-induced cell damage in vitro. (a and b) Expression of IL-22R in human and rat bile duct tissues and HIBEpiCs. CK19: a marker of bile duct epithelial cells. THP-1 served as a negative control for IL-22R expression. MHCC-97h served as a positive control. (c) The inhibitory effect of CoCl2 on HIBEpiCs activity after being treated with 0~300 μM for 24 h. (d) Cell viability at different time points treated with 150 μM CoCl2. (e) HIF-1α protein expression with increasing CoCl2 concentration. (f) Viability of HIBEpiCs after treatment with 0~200 ng/ml IL-22 for 24 h. (g) The effect of 10 ng/ml IL-22 on 150 μM CoCl2-induced HIBEpiCs injury at 24, 48, and 72 hours. Data are shown as . .
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