Ferrostatin-1 and 3-Methyladenine Ameliorate Ferroptosis in OVA-Induced Asthma Model and in IL-13-Challenged BEAS-2B Cells

Figure 3

Ferr-1 and 3-MA alleviated the inflammation and oxidative damage of lung tissues in asthma mice. (a) H&E staining was used for the detection of the damage of lung tissues. (b) The expression of LC-3 in lung tissues was detected with immunohistochemistry. (c) The levels of IL-4, IL-5, and IL-13 in lung tissues and BALF were determined with ELISA. (d) The levels of IL-1β, IL-6, and TNF-α in lung tissues and BALF were determined with ELISA. (e) The levels of ROS, SOD, and MDA in lung tissues and BALF were detected with commercial kits. vs. the control; ^#, ^##, and ^### vs. the Model. Ferr-1: ferrostatin-1; 3-MA: 3-methyladenine; BALF: bronchoalveolar lavage fluid.

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