Ferrostatin-1 and 3-Methyladenine Ameliorate Ferroptosis in OVA-Induced Asthma Model and in IL-13-Challenged BEAS-2B Cells

Figure 5

Ferr-1 and 3-MA relieved lipid peroxidation in lung tissues in asthma mice. (a–c) The level of GSH and GSSG was determined with the commercial kits. (d) The expression of GPX4, SLC7A11, SLC3A2, and ALOX5 in lung tissues was determined with western blotting. (e) The expression of NCOA4, TFR1, and DMT-1 in lung tissues was detected with western blotting. (f) The expression of ATG5 and ATG7 in lung tissues was determined with western blotting. vs. the control; ^#, ^##, and ^### vs. the model. Ferr-1: ferrostatin-1; 3-MA: 3-methyladenine; GSH: reduced glutathione; GSSG: oxidized glutathione; GPX4: glutathione peroxidase 4; SLC7A11: solute carrier family 7 member 11; SLC3A2: solute carrier family 3 member 2; ALOX5: arachidonate-5-lipoxygenase; NCOA4: nuclear receptor coactivator 4; TFR1: transferrin receptor; DMT-1: divalent metal transporter 1; ATG5: autophagy-related 5; ATG7: autophagy-related 7.

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