Ferrostatin-1 and 3-Methyladenine Ameliorate Ferroptosis in OVA-Induced Asthma Model and in IL-13-Challenged BEAS-2B Cells

Figure 6

ALI and IL-13 induced the damage of bronchial epithelial cells. (a) CCK-8 was performed for the detection of the viability of these cells. (b–d) The levels of IL-1β, IL-6, and TNF-α in these cells were determined with ELISA. (e–g) The levels of ROS, SOD, and MDA in these cells were detected with commercial kits. (h) The expression of LC-3 in these cells was detected with immunofluorescence. , , and vs. the control; ^#, ^##, and ^### vs. ALI. ALI: air-liquid interface; CCK-8: cell counting kit-8; ROS: reactive oxygen species; SOD: superoxide dismutase; MDA: malonaldehyde.

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