Ferrostatin-1 and 3-Methyladenine Ameliorate Ferroptosis in OVA-Induced Asthma Model and in IL-13-Challenged BEAS-2B Cells

Figure 9

Ferr-1 and 3-MA relieved iron deposition, lipid peroxidation, and ferritinophagy in IL-13-induced BEAS-2B cells. (a–c) The levels of free iron ions in these cells were detected with commercial kits. (d–f) The ratio of GSH/GSSG was determined with the commercial kits. (g) The expression of GPX4, SLC7A11, SLC3A2, and ALOX5 in these cells was determined with western blotting. (h) The expression of NCOA4, TFR1, and DMT-1 in these cells was detected with western blotting. (i) The expression of ATG5 and ATG7 in these cells was determined with western blotting. vs. the control; ^#, ^##, and ^### vs. ALI+IL-13. Ferr-1: ferrostatin-1; 3-MA: 3-methyladenine; ALI: air-liquid interface; GSH: reduced glutathione; GSSG: oxidized glutathione; GPX4: glutathione peroxidase 4; SLC7A11: solute carrier family 7 member 11; SLC3A2: solute carrier family 3 member 2; ALOX5: arachidonate-5-lipoxygenase; NCOA4: nuclear receptor coactivator 4; TFR1: transferrin receptor; DMT-1: divalent metal transporter 1; ATG5: autophagy-related 5; ATG7: autophagy-related 7.

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