[Retracted] Exploring the Temporal Correlation of Sarcopenia with Bone Mineral Density and the Effects of Osteoblast-Derived Exosomes on Myoblasts through an Oxidative Stress–Related Gene

Figure 5

Exosomes derived from skull osteoblasts or femur osteoblasts exert different effects on myoblasts. (a) Representative images of purified skull osteoblast– or femur osteoblast–derived exosomes detected by TEM. Scale bars: 100 nm. (b) Purified exosomes derived from skull osteoblasts or femur osteoblasts were identified by nanoparticle tracking analysis. (c) WB analysis of exosomal markers CD63 and CD81 in exosomes derived from skull osteoblasts or femur osteoblasts. (d) The proliferation rate of C2C12 cells detected by CCK8 array. (e) Representative images of flow cytometric apoptosis assay in C2C12 cells. The bar graph shows the quantification of apoptotic cell number in each group. (f) The protein levels of Myog and MYH6 in C2C12 cells detected using WB. The bar graph shows the relative levels of Myog and MYH6 in each group. CM: C2C12 cell culture medium; DM: differential medium; Exo, exosomes. . and .

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