[Retracted] Exploring the Temporal Correlation of Sarcopenia with Bone Mineral Density and the Effects of Osteoblast-Derived Exosomes on Myoblasts through an Oxidative Stress–Related Gene

Figure 9

lncRNA TUG1 and DANCR regulate Myog expression via modifying nuclear translocation of Myf5 in myoblasts. (a) The level of lncRNA TUG1 and DANCR binding to Myf5 protein detected by RIP assay. (b) Myog gene promoter activity analyzed by relative luciferase reporter activities in C2C12 cells transfected with lncRNA TUG1 expression vector, lncRNA DANCR expression vector, or Myf5 expression vector. (c) Quantification of Myf5 occupancy on the Myog gene promoter by ChIP-qRT-PCR in C2C12 cells transfected with lncRNA TUG1 expression vector or lncRNA DANCR expression vector. (d) Representative images of C2C12 cells staining by Myf5 antibody using IF. The bar graph shows the quantification of mean fluorescence intensity (MFI) of red in the nucleus.  μm. OE: overexpression. . and .

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