Changes of Functional, Morphological, and Inflammatory Reactions in Spontaneous Peripheral Nerve Reinnervation after Thermal Injury

Figure 5

Macro- and micromorphological changes of gastrocnemius muscles at 30 days after thermal injury. (a) Gross observation of gastrocnemius muscles. (b) Comparison of the weight changes in the four groups; the order of weights of the injured limb are listed as follows: with significant difference. (c) Recording the morphology of muscle cells by microscopic observation (H&E staining, 100x). (d) Getting the number of NMJ in gastrocnemius muscles by IF staining (indexes: neurofilament and DAPI). Nuclei were stained blue using DAPI, and neural fibers stained red using antineurofilament. represents . NMJ: neuromuscular junctions; IF: immunofluorescent.

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