Forsythiaside A Regulates Activation of Hepatic Stellate Cells by Inhibiting NOX4-Dependent ROS

Figure 9

FA inhibited ROS production and HSCs activation partly via inhibiting NOX4 over-expression. (A) SiRNA concentration screening. p <0.001 siRNA-NOX4 group vs control; p <0.01 siRNA-NOX4 group vs control; p <0.05 siRNA-NOX4 group vs control. (B-C) ROS levels measured by an inverted fluorescence microscope (100X) and quantified by ImageJ software. (D-G) The proteins expression of NOX4, α-SMA and COL I. p <0.001 vs siRNA-control; p <0.01 vs siRNA-control; p <0.05 vs siRNA-control.^###p <0.001 vs siRNA-control + TGF-β1;^##p <0.01 vs siRNA-control + TGF-β1.

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