Circular RNA_0057209 Acts as ceRNA to Inhibit Thyroid Cancer Progression by Promoting the STK4-Mediated Hippo Pathway via Sponging MicroRNA-183

Figure 3

High expression of circRNA_0057209 inhibited the proliferation, migration, and invasion of thyroid cancer cells. (a) The relative expression of circRNA_0057209 in oe-circRNA_0057209-treated TPC-1 and HTH83 cells determined by qRT-PCR. (b) CCK-8 showing the proliferation of oe-circRNA_0057209-treated TPC-1 cells. (c) CCK-8 showing the proliferation of oe-circRNA_0057209-treated HTH83 cells. (d) Transwell assay showing the migration of oe-circRNA_0057209-treated TPC-1 and HTH83 cells. (e) Transwell assay showing the invasion of oe-circRNA_0057209-treated TPC-1 and HTH83 cells. (f) Cell cycle distribution upon circRNA_0057209 overexpression analyzed using FCM. (g) Apoptosis rate of oe-circRNA_0057209-treated TPC-1 and HTH83 cells assessed using FCM. (h) Western blot showing the relative expression of E-cadherin, N-cadherin, Ki67, PCNA, Bax, and Bcl-2 in oe-circRNA_0057209-treated TPC-1 and HTH83 cells. compared with the oe-NC group. The cell experiment was repeated three times independently.

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