Circular RNA_0057209 Acts as ceRNA to Inhibit Thyroid Cancer Progression by Promoting the STK4-Mediated Hippo Pathway via Sponging MicroRNA-183

Figure 4

circRNA_0057209 competitively binds to miR-183 to reduce its expression in thyroid cancer cells. (a) Predicted binding sites of circRNA_0057209 and miR-183. (b) FISH analysis of circRNA_0057209 colocalization with miR-183 cells. The blue part in the figure represents the nucleus, the green part represents circRNA_0057209, and the red part represents miR-183 (×400). (c) The expression of miR-183 in thyroid cancer tissues and adjacent normal tissues determined by qRT-PCR, , , , . (d) Correlation analysis between circRNA_0057209 and miR-183. (e) The expression of miR-183 in four kinds of thyroid cancer cells and normal thyroid cells determined by qRT-PCR. Compared with NTHY-ORI3.1 cells, , , . (f) The expression of miR-183 in cells treated with oe-circRNA_0057209 determined by qRT-PCR. , , . (g) Dual luciferase reporter gene assay for identification of the interaction between circRNA_0057209 and miR-183 upon treatment with oe-NC or oe-circRNA_0057209. Compared with the NC mimic group , , . (h) RIP assay determining the binding of circRNA_0057209, miR-183 and Ago2 or IgG. Compared with the input group and ^# indicated compared with the IgG group, , , ; ^##, ^###. (i) Interaction between miR-183 and circRNA_0057209 analyzed by RNA pull-down assay. compared with the NC-biotin group, , . The cell experiment was repeated three times independently.

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