Circular RNA_0057209 Acts as ceRNA to Inhibit Thyroid Cancer Progression by Promoting the STK4-Mediated Hippo Pathway via Sponging MicroRNA-183

Figure 5

Upregulation of miR-183 abrogated the tumor-suppressive effect of circRNA_0057209 on thyroid cancer. Thyroid cancer cells were treated with oe-circRNA_0057209 or combined with miR-183 mimic. (a) The expression of circRNA_0057209 and miR-183 in thyroid cancer cells determined by qRT-PCR. (b) CCK-8 showing thyroid cancer cell proliferation. (c) Transwell assay showing thyroid cancer cell migration. (d) Transwell assay showing cell invasion. (e) FCM images of thyroid cancer cell cycle distribution. (f) FCM showing cell apoptosis rate. (g) Western blot assay showing the relative protein expression of E-cadherin, N-cadherin, Ki67, PCNA, Bax, and Bcl-2 in thyroid cancer cells. Compared with the oe-NC+NC mimic group, ^# indicated that compared with the oe-circRNA_0057209+NC mimic group, , , ; ^##, ^###. The cell experiment was repeated three times independently.

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