Circular RNA_0057209 Acts as ceRNA to Inhibit Thyroid Cancer Progression by Promoting the STK4-Mediated Hippo Pathway via Sponging MicroRNA-183

Figure 6

circRNA_0057209 acted as ceRNA of miR-183 to upregulate the expression of STK4 (a) The predicted binding site between miR-183 and STK4 by bioinformatics analysis. (b) Dual luciferase activity detection to verify the binding between miR-183 and STK4. (c) STK4 expression in thyroid cancer tissues and adjacent normal tissues determined by qRT-PCR. (d) Representative images of IHC of STK4-positive cells thyroid cancer tissues and adjacent normal tissues (×200). (e) Correlation analysis between circRNA_0057209 and STK4, miR-183, and STK4. (f) The expression of STK4 in BCPAP, TPC-1, IHH-4, HTH83, and NTHY-ORI3.1 cells determined by qRT-PCR. (g) The mRNA expression of STK4 in cells upon treatment with oe-circRNA_0057209 or combined with miR-183 mimic determined by qRT-PCR. (h) Western blot assay showing the relative protein expression of STK4 in cells upon treatment with oe-circRNA_0057209 or combined with miR-183 mimic. Compared with the oe-NC+NC mimic group, , ^# indicated that compared with the oe-circRNA_0057209+NC mimic group, , , ; ^##, ^###. The cell experiment was repeated three times independently.

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