Thyroid Cancer-Associated Mitochondrial DNA Mutation G3842A Promotes Tumorigenicity via ROS-Mediated ERK1/2 Activation

Figure 4

Effects of the G3842A mutation on mitochondrial function. (a) Measurements of the content of mitochondrial complexes I, II, III, IV, and V in WT and MUT using BN-PAGE and immunoblotting. (b) Measurements of complex I-dependent oxygen respiration in WT and MUT. (c) The OCR of WT and MUT was determined using an XF24 extracellular flux analyzer and initially under basal conditions, followed by the addition of oligomycin (100 μg/mL) and FCCP (50 μg/mL). Measurements of (d) ATP levels and (e) MMP levels in WT and MUT cells. The OCR and ATP generation were normalized against protein concentrations. Data are presented as of at least three replicates. , , and . BN-PAGE: blue native polyacrylamide gel electrophoresis; OCR: oxygen consumption rate; ATP: adenosine triphosphate; MMP: mitochondrial membrane protein; FCCP: carbonyl cyanide-p-trifluoromethoxy phenyl hydrazine.

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