Thyroid Cancer-Associated Mitochondrial DNA Mutation G3842A Promotes Tumorigenicity via ROS-Mediated ERK1/2 Activation

Figure 5

Effects of the G3842A mutation on messenger molecules. ROS levels are indicated by the relative levels of fluorescence intensity of MitoSOX (a) and DCF (b) in WT and MUT cells. Ca²⁺ (c) and the NAD/NADH ratio (d) were determined in WT and MUT cells. Signals for WT, MUT, and blank are shown in blue, red, and white, respectively. The horizontal coordinate corresponding to the signal peak represents the fluorescence intensity. We defined mean fluorescence intensity of WT as “1”: . Relative fluorescence intensity was obtained by comparing the fluorescence intensity of MUT (after subtracting the fluorescence intensity of the blank) with the mean fluorescence intensity of WT, for example, . Data are presented as of at least three replicates. , , and . ROS: reactive oxygen species; NADH: nicotinamide adenine dinucleotide hydride.

(a)

(b)

(c)

(d)