Thyroid Cancer-Associated Mitochondrial DNA Mutation G3842A Promotes Tumorigenicity via ROS-Mediated ERK1/2 Activation

Figure 7

The G3842A mutation promotes tumorigenicity via increased ROS generation and subsequent ERK1/2 phosphorylation. (a) Measurements of p-ERK1/2 levels in WT and MUT treated with NAC (10 mM for 24 or 48 h) or without NAC using western blot. (b) Colony formation assays of WT and MUT treated with or without NAC. (c) The migration abilities of WT and MUT treated with or without NAC were test using Transwell assays. (d) p-ERK1/2 levels in WT and MUT treated with U0126 (25 or 50 μm for 2 or 12 h) or without U0126 were evaluated by Western blot. (e) Colony formation assays and (f) Transwell assays of WT and MUT treated with or without U0126. Data are presented as of at least three replicates. , , and . NAC: N-acetyl-L-cysteine.

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