Anlotinib Alleviates Renal Fibrosis via Inhibition of the ERK and AKT Signaling Pathways

Figure 2

Anlotinib reduced the accumulation of extracellular matrix components and blocked EMT in UUO mice. (a) Quantitative RT-PCR was performed to determine the RNA expression of α-SMA, TGF-β1, collagen І, and collagen ІV in the kidney tissue of UUO mice. (b) Western blotting was performed to determine the protein expression of α-SMA, collagen І, and collagen ІV in the kidney tissue of UUO mice. (c) The representative photographs of the effects of anlotinib against OUU-induced pathological changes (HE and Masson stain) at day 14; interstitial fibrosis was evident in mouse kidney from the UUO group, compared with the sham group. The pathological changes were obviously relieved in the kidney of the anlotinib-treated group. This was immunohistochemical staining of α-SMA in the renal cortex from the experimental mice. (Bars, 100 μm. All data are represented as . and , . Scale bars, 100 μm.).

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