P16^INK4a Regulates ROS-Related Autophagy and CDK4/6-Mediated Proliferation: A New Target of Myocardial Regeneration Therapy

Figure 2

P16^INK4a regulates the proliferation of NMCMs in vitro. (a, b) Cardiomyocytes were transfected with Ad5:cTnT-CON (NC), Ad5:cTnT-INK4a (INK4a), or Ad5:cTnT-INK4a RNAi (INK4ai), and p16^INK4a expression was detected by Western blot and qRT-PCR (). (c–f) Cardiomyocyte proliferation is quantified by immunofluorescence for Ki67, pH 3, EdU, and Aurora B in cardiomyocytes transfected with INK4ai/NC/INK4a (). Scale bar: 50 μm. (g) The G0/1, S, and G2/M phase proportions of INK4ai/NC/INK4a cardiomyocytes were detected by flow cytometry and cell cycle analysis () (data are presented as ; NS: no statistical difference, , , ).

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