P16^INK4a Regulates ROS-Related Autophagy and CDK4/6-Mediated Proliferation: A New Target of Myocardial Regeneration Therapy

Figure 3

Knockdown p16^INK4a prolongs the period of myocardial regeneration in neonatal mice in vivo. (a) Western blot analysis was performed to detect the expression of p16^INK4a in the myocardium at P7 after intraperitoneal injection of Ad5:cTnT-CON (NC) or Ad5:cTnT-INK4a RNAi (INK4ai). (b) Cardiac appearance and the ratio of heart weight and body weight (HW/BW) in mice at P28 after intraperitoneal injection of NC or INK4ai (). (c) WGA staining was used to count the cardiomyocyte size changes at P28 after intraperitoneal injection of NC or INK4ai (). Scale bar: 50 μm. (d, e) The proliferation proportion of cardiomyocytes in the heart at P7 was counted by Ki67 and pH 3 immunofluorescence after intraperitoneal injection of NC or INK4ai in neonatal mice (). Scale bar: 20 μm, 50 μm (data are presented as ; NS: no statistical difference, , ).

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