P16^INK4a Regulates ROS-Related Autophagy and CDK4/6-Mediated Proliferation: A New Target of Myocardial Regeneration Therapy

Figure 5

Proteomics analysis shows that knockdown P16 activates cell cycle processes and energy metabolism balance. (a) Volcano plot of upregulated, downregulated, and unchanged proteins in the Ad5:cTnT-INK4a RNAi (INK4ai) group versus the Ad5: cTNT-CON (NC) group; multiples change greater than 1.5 times and value < 0.05. (b) Heatmap of cluster analysis of differentially expressed proteins among cardiomyocytes treated with INK4ai or NC. (c) Histograms of the frequency of KOG functional classifications, with the abscissa representing the functional annotation of each bar classification, indicated by an alphabetical abbreviation; the ordinate indicates the number of proteins corresponding to each classification. (d) GO secondary classification histograms; the ordinate indicates the enriched GO functional classification into BP, CC, and MF. The abscissa represents the -log10 ( value) value corresponding to each entry. (e) KEGG functional enrichment bubble plot; the colour gradient represents the size of the value, and the bubble size represents the number of differentially expressed proteins involved in this KEGG pathway.

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