RNF7 Facilitated the Tumorigenesis of Pancreatic Cancer by Activating PI3K/Akt Signaling Pathway

Figure 3

Downregulated RNF7 reduced the proliferation, invasion, and migration of PC cells. (a) Relative expression of RNF7 in PC cells (SW1990 and PANC-1) following transfection of shRNA1, shRNA2, and negative control (sh-NC). (b, c) CCK-8 analysis of the cell proliferative potential after the transfection with RNF7 shRNAs treatment in SW1990 and PANC-1 cell lines. (d, e) The EdU assays analyzed PC proliferative property (SW1990 and PANC-1). (f, g) Transwell analysis of the cell invasive potential after the transfection with RNF7 shRNAs treatment in SW1990 and PANC-1 cell lines. (h, i) Wound-healing analysis of the cell migrative potential after the transfection with RNF7 shRNAs treatment in SW1990 and PANC-1 cell lines (scar bar: 50 μm). ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)