RNF7 Facilitated the Tumorigenesis of Pancreatic Cancer by Activating PI3K/Akt Signaling Pathway

Figure 5

The rescue experiment of the effect of RNF7 on proliferation, migration, and invasion of PC cells. (a, b) Cell proliferation assay of SW1990 and PANC-1 cells. Overexpression of RNF7 promoted the proliferation ability and the inhibitor of perifosine inhibited this effect. (c–f) Transwell assay of SW1990 and PANC-1 cells. Overexpression of RNF7 promoted the invasion ability and the inhibitor of perifosine inhibited this effect (scar bar: 50 μm). (g) Protein expression levels and quantitative data of active phospho-Akt and phospho-mTOR in SW1990 and PANC-1 cells were shown. Overexpression of RNF7 activated the PI3K/AKT pathway, and the signaling inhibitor perifosine counteracted the activation.

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