Aldehyde Dehydrogenase 2 Protects the Kidney from Ischemia–Reperfusion Injury by Suppressing the IκBα/NF-κB/IL-17C Pathway

Figure 1

ALDH2 deficiency exacerbates renal ischemia–reperfusion injury. WT mice and ALDH2^-/- mice were subjected to either sham operation or kidney ischemia for 45 minutes and reperfusion for 24 hours. (a) Kidney lysates from WT mice and ALDH2^-/- mice were subjected to western blot analysis for ALDH2. /group. (b) An illustration of the injury model used in this study. (c) Serum creatinine (SCr) were measured after 24 h of reperfusion. /group. (d) H&E staining showed renal tubule epithelial cell injury and red blood cell deposition. Scale bar: 200 μm, 50 μm. (e) Paller score was performed using a semiquantitative damage assessment of renal tubular epithelial cells for each sample. /group. (f) Representative images of TUNEL staining (green) and DAPI (blue). Scale bar: 100 μm. (g) The percentage of TUNEL staining-positive cells in the total cells in the random field. /group. (h) Morphology of renal tubular epithelial cell was observed under TEM. /group. Scale bar: 20 μm, 10 μm. Data are presented as the . , , and by -test.

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