Aldehyde Dehydrogenase 2 Protects the Kidney from Ischemia–Reperfusion Injury by Suppressing the IκBα/NF-κB/IL-17C Pathway

Figure 4

Activation of ALDH2 reduced inflammation and inhibited the phosphorylation of IκBα/NF-κB p65 in I/R. (a)–(c) The expression of IL-10β, MCP-1, and TNF-α mRNA in the kidney was determined by real-time PCR. GAPDH was the standard. /group. (d) The western blot band of p-IκBα, IκBα, p-p65, p65, and IL-17C in kidney lysates. β-Actin was the standard. /group. (e)–(g) The western blot analysis of p-IκBα, IκBα, p-p65, p65, and IL-17C protein in kidney samples from each group. /group. Data are presented as the . , , and by -test.

(a)

(b)

(c)

(d)

(e)

(f)

(g)