Aldehyde Dehydrogenase 2 Protects the Kidney from Ischemia–Reperfusion Injury by Suppressing the IκBα/NF-κB/IL-17C Pathway

Figure 5

Activation of ALDH2 alleviates kidney ischemia–reperfusion injury, while inhibition aggravates the injury. WT mice were pretreated with solvent, CYA, and Alda-1, followed by kidney ischemia for 45 minutes and reperfusion for 24 hours. (a) Kidney lysates from WT mice after medical preconditioning were subjected to enzyme assay for ALDH2. . (b) Kidney lysates from WT mice were subjected to western blot analysis for ALDH2. /group. (c) Western blot analysis for ALDH2 protein in kidney samples from each group. /group. (d) Schematic diagram of Alda-1 and CYA preconditioning with ischemia–reperfusion (e) Serum creatinine (SCr) were measured after 24 h of reperfusion. . (f) H&E staining showed the injury of renal tubular epithelial cells and accumulation of red blood cells. Scale bar: 200 μm, 50 μm. (g) Paller score was performed using a semiquantitative damage assessment of renal tubular epithelial cells for each sample. . (h) Representative images of TUNEL staining (green) and DAPI (blue). Scale bar: 100 μm. (i) The percentage of TUNEL staining-positive cells in the total cells in the random field. /group. (j) Morphology of renal tubular epithelial cell was observed under TEM. /group. Scale bar: 20 μm, 10 μm. Data are presented as the . , , and by -test.

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