Aldehyde Dehydrogenase 2 Protects the Kidney from Ischemia–Reperfusion Injury by Suppressing the IκBα/NF-κB/IL-17C Pathway

Figure 6

NF-κB phosphorylase inhibitors attenuate the aggravation of injury caused by ALDH2 deficiency. (a) The western blot analysis of ALDH2 in HK-2 lysates. β-Actin was the standard. /group. (b) Kidney lysates from WT mice were subjected to western blot analysis for ALDH2. /group. (c) Cell lysates from HK-2 cell after knockdown ALDH2 and JSH-23 preconditioning were subjected to enzyme assay for ALDH2. /group. (d) Schematic diagram of HK-2 cell treatment. (e) The apoptosis of HK-2 cells was measured by flow cytometry, and the statistical results were expressed by histogram (f). /group. (g) The western blot band of p-IκBα, IκBα, p-p65, p65, and IL-17C in kidney lysates. β-Actin was the standard. /group. (h)–(j) Western blot analysis for p-IκBα, IκBα, p-p65, p65, and IL-17C protein in HK-2 cell samples from each group. /group. Data are presented as the . n.s.: not significant. , , and by -test.

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