Microbiota from Exercise Mice Counteracts High-Fat High-Cholesterol Diet-Induced Cognitive Impairment in C57BL/6 Mice

Figure 4

Tau phosphorylation, synaptic function, and neuroinflammation-related markers from hippocampus. (a) Protein expression including p-tau ser396 and 404, BDNF, PSD95, and synaptophysin of hippocampus was measured by western blot. (b) TNF-α and IL-6 mRNA expressions from hippocampus were measured by qPCR. (c) Representative double immunofluorescence images of GFAP (in green) and Ibα1 (in red) from mouse cerebral cortex (×50 objective). AQP4 protein expression was measured by western blot (d) and immunofluorescence (e) (×50 objective), respectively. Data were presented as (). versus CON and ^# versus FMTSED.

(a)

(b)

(c)

(d)

(e)