Osthole Inhibits M1 Macrophage Polarization and Attenuates Osteolysis in a Mouse Skull Model

Figure 7

OST protected mice against LPS-induced inflammatory osteolysis in vivo. (a) Representative three-dimensional micro-CT reconstructions of murine calvaria from each treatment group. Collagen sponges soaked with PBS (sham) or LPS (10 mg/kg body weight) were implanted over the mouse calvarial bones, and PBS or OST (1.5 or 7.5 mg/kg) was injected subcutaneously over the sagittal midline suture of the calvaria over a 14-day period. . (b, c) Morphometric analysis of the percentage of bone volume to tissue volume (, %) and percentage of total porosity was conducted. (d) Histomorphometric analysis of the number of TRAP-positive osteoclasts in the calvarial bone sections. (, , ). (e) Representative hematoxylin and eosin- (×50 or 100) and TRAP- (×100) stained sections from each treatment group. , 200 μM(×100). Data are expressed as median and interquartile range, .

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