[Retracted] Active Ingredient Paeonol of Jijiu Huiyang Decoction Alleviates Isoproterenol-Induced Chronic Heart Failure via the GSK3A/PPARα Pathway

Figure 5

Paeonol downregulated GSK3A and relieved myocardial hypertrophy and cardiac lipid accumulation in CHF mice. (a) Representative hematoxylin-eosin staining of mouse hearts from the five groups (magnification, 40×, scale  μM). The mRNA levels of (b) ANP, (c) BNP, and (d) B-MHC (markers of myocardial hypertrophy) determined by qRT-PCR, taking GAPDH as the internal control. (e) Macromolecular docking diagram of Pae and GSK3A. (f) The protein levels of GSK3A and (g) corresponding quantitative bar chart determined by western blot. (h) The mRNA levels of GSK3A determined by qRT-PCR, taking GAPDH as the internal control. (i) Representative Oil red O staining of cardiac lipid accumulation from the five groups and (j) corresponding histological slides (magnification, 200×, scale  μM). ^{^}vs. control group. vs. Pae-50 group. ^###vs. CHF group. Pae: paeonol; CHF: chronic heart failure; GSK3A: Glycogen Synthase Kinase-3 alpha; ANP: atrial natriuretic peptide; BNP: B-type natriuretic peptide; B-MHC: major histocompatibility complex-B; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

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