[Retracted] Active Ingredient Paeonol of Jijiu Huiyang Decoction Alleviates Isoproterenol-Induced Chronic Heart Failure via the GSK3A/PPARα Pathway

Figure 6

Overexpression of GSK3A reversed the inhibitory effect of paeonol on ISO-induced lipid accumulation in human cardiomyocytes. CCC-HEH-2 cells receiving different concentrations of Pae for 30 minutes, (a) the GSK3A protein expression detected by western blot and (b) their corresponding quantitative bar chart, taking GAPDH as the internal control. (c, d) CCC-HEH-2 cells pretreated with or without Pae (100, 400 μM) for 30 minutes and then subjected to a 24 hours of incubation with ISO (10 μM), and the GSK3A expression level assessed by western blot. (e, f) The lipid accumulation detected by Oil red O staining (magnification, 200×, scale  μM). (g, h) CCC-HEH-2 cells transfected with GSK3A overexpression plasmid or NC pretreated with or without Pae (400 μM) for 30 minutes and then subjected to a 24 hours of incubation with ISO (10 μM), and the GSK3A expression level determined by western blot, (i, j) the lipid accumulation examined by Oil red O staining (magnification, 200×, scale  μM). , vs. blank group. ^#, ^###vs. ISO group. ^&&&vs. ISO + Pae-400 group. Pae: paeonol; GSK3A: Glycogen Synthase Kinase-3 alpha; ISO: isoproterenol; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; NC: negative control.

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