[Retracted] Active Ingredient Paeonol of Jijiu Huiyang Decoction Alleviates Isoproterenol-Induced Chronic Heart Failure via the GSK3A/PPARα Pathway

Figure 7

Overexpression of GSK3A reversed paeonol’s inhibitory effect on ISO-induced PPARα pathway activation. (a–d) CCC-HEH-2 cells pretreated with or without Pae (100, 400 μM) for 30 minutes and then subjected to a 24 hours of incubation with ISO (10 μM), and the p-PPARα (b), PPARα (c) and the ratio of p-PPARα/PPARα (d) protein levels measured by western blot. (e–h) Taking GAPDH as the internal control, CCC-HEH-2 cells transfected with GSK3A overexpression plasmid or NC pretreated with or without Pae (400 μM) for 30 minutes and then subjected to a 24 hours of incubation with ISO (10 μM), and the p-PPARα, PPARα, and the ratio of p-PPARα/PPARα protein levels determined by western blot. (i) the mRNA levels of CD36, Cpt1b, and PDK4 (PPARα downstream target genes) obtained by qRT-PCR. (j) the mRNA levels of CD36, Cpt1b, and PDK4 (PPARα downstream target genes) determined by qRT-PCR, with GAPDH being the internal control. vs. blank group. ^###vs. ISO group. ^&&&vs. ISO + Pae-400 group. Pae: paeonol; GSK3A: Glycogen Synthase Kinase-3 alpha; ISO: isoproterenol; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; NC: negative control; Cpt1b: carnitine palmitoyltransferase 1B; PDK4: pyruvate dehydrogenase kinase 4.

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