[Retracted] Active Ingredient Paeonol of Jijiu Huiyang Decoction Alleviates Isoproterenol-Induced Chronic Heart Failure via the GSK3A/PPARα Pathway

Figure 8

SiPPARα reversed the regulation of GSK3A overexpression on the PPARα pathway activation, lipid accumulation, and apoptosis. (a–d) CCC-HEH-2 cells transfected with NC or GSK3A overexpression plasmid or cotransfected with GSK3A overexpression plasmid/NC and siPPARα, the p-PPARα and PPARα protein levels as well as the ratio of p-PPARα/PPARα measured by western blot. (e) The mRNA levels of CD36, Cpt1b, and PDK4 (PPARα downstream target genes) assessed by qRT-PCR. (f, g) The lipid accumulation detected by Oil red O staining (magnification, 200×, scale  μM). (h, i) The apoptosis rate determined by flow cytometry. vs. NC group. ^##, ^###vs. GSK3A group. ^{^}, ^{^^}, ^{^^^}vs. NC+ siPPARα group. Pae: paeonol; GSK3A: Glycogen Synthase Kinase-3 alpha; ISO: isoproterenol; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; NC: negative control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)