MBNL1-AS1 Promotes Hypoxia-Induced Myocardial Infarction via the miR-132-3p/RAB14/CAMTA1 Axis

Figure 4

miR-132-3p directly targets CAMTA1 and RAB14. (a) Two mRNAs (CAMTA1 and RAB14) targeted by miR-132-3p were sifted out by four bioinformatics tools including PITA, miRmap, microT, and RNA22. (b) Expression of CAMTA1 and RAB14 were detected in H9c2 cells under hypoxia treatment for 0-12 h. (c) RIP assays detected the abundance of MBNL1-AS1, miR-132-3p, CAMTA1, and RAB14 in anti-Ago2 groups. (d, e) Binding sites between miR-132-3p and RAB14 3UTR/CAMTA1 3UTR, and luciferase activities of RAB14 3UTR-WT/MUT as well as CAMTA1 3UTR-WT/MUT were assessed in hypoxia-treated H9c2 and HEK293T cells after miR-132-3p overexpression. (f, g) Expression of RAB14 and CAMTA1 were examined in hypoxia-treated H9c2 cells after the transfection of sh/NC, sh/MBNL1-AS1#1, and sh/MBNL1-AS1#1 + miR-132-3p inhibitor, respectively. .

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