Diabetes-Induced Autophagy Dysregulation Engenders Testicular Impairment via Oxidative Stress

Figure 7

Expression of HIF-1α, LC-3I/II, p62, and cleaved caspase-3 during AGE-induced oxidative stress in Sertoli cells. Sertoli cells were treated with HG (20 mM) or AGEs (100 μg/ml) for 72 h. AGEs aggravated HIF-1α expression and induced autophagy dysregulation. (a) Representative immunoblotting of HIF-1α, LC-3I/II, p62, Beclin1, and cleaved caspase-3 in Sertoli cells treated with HG and AGEs. (b) Densitometric qualification of HIF-1α, LC-3II, and Beclin1 in Sertoli cells treated with HG and AGEs. (c) Densitometric qualification of p62 and cleaved caspase-3 in Sertoli cells treated with HG and AGEs. was considered to indicate a statistically significant difference. vs. Ctrl. ^# vs. HG.

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