The Role of the miR-548au-3p/CA12 Axis in Tracheal Chondrogenesis in Congenital Pulmonary Airway Malformations

Figure 4

CA12 as a direct target of miR-548au-3p. (a) Binding sites between miR-548au-3p and the 3-UTR region of CA12 mRNA. (b) HEK-293 cells were cotransfected with miR-548au-3p mimics or NC and wild-type or mutant CA12 3-UTR, followed by luciferase reporter assay. CA12 mRNA (c) and protein (d) levels in rat tracheal chondrocytes after transfection with miR-548au-3p mimics or inhibitor. and , compared with NC.

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