Research Article
The Role of the miR-548au-3p/CA12 Axis in Tracheal Chondrogenesis in Congenital Pulmonary Airway Malformations
Table 1
Primers for real-time PCR.
| | Name of genes | Sequences (5ā3) |
| | GAPDH F | TGTTCGTCATGGGTGTGAAC | | GAPDH R | ATGGCATGGACTGTGGTCAT | | CA12 F | AGGCCAGGAAGCATTCGTC | | CA12 R | GGGAAGGGTCGTCCATGTG | | U6 F | CTCGCTTCGGCAGCACA | | U6 R | AACGCTTCACGAATTTGCGT | | All R | CTCAACTGGTGTCGTGGA | | miR-146a-5p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAACCCATG | | miR-146a-5p F | ACACTCCAGCTGGGTGAGAACTGAATTCCA | | miR-590-3p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACTAGCTT | | miR-590-3p F | ACACTCCAGCTGGGTAATTTTATGTATAA | | miR-21-3p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACAGCCCA | | miR-21-3p F | ACACTCCAGCTGGGCAACACCAGTCGATG | | miR-548au-3p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCTGGTGCA | | miR-548au-3p F | ACACTCCAGCTGGGTGGCAGTTACTTTTG | | miR-4523 RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACAGCCGA | | miR-4523 F | ACACTCCAGCTGGGGACCGAGAGGGCCTC |
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F: forward primer; R: reverse primers; RT: reverse transcription.
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