Olive Leaves Extract and Oleuropein Improve Insulin Sensitivity in 3T3-L1 Cells and in High-Fat Diet-Treated Rats via PI3K/AkT Signaling Pathway

Table 1

Primers for RT-PCR. PCR was performed using the primers indicated as below under optimal amplification condition (95°C for 5 min; 22–35 cycles of 95°C for 30 s, 58°C for 30 s, 72°C for 30 s; and 72°C for 7 min) for each gene.

Name	Forward	Reverse
GAPDH	5-TGGTGAAGGTCGGTGTGAACGG-3	5-TGCCGTTGAATTTGCCGTGAGT-3
PPARγ	5-AAACTCTGGGAGATTCTCCT-3	5-TGGCATCTCTGTGTCAAC-3
C/EBPα	5-GCCAAACTGAGACTCTTC-3	5-TGGCATCTCTGTGTCAAC-3
SREBP-1c	5-GCTTAGCCTCTACACCAACTGGC-3	5-ACAGACTGGTACGGGCCACAAG-3
FAS	5-TGGAGCCTGTGTAGCCTTCGAG-3	5-ACAGCCTGGGGTCATCTTTGCC-3
Il-6	5-GGTGACAACCACGGCCTTCCC-3	5-GCCACTCCTTCTGTGACTCCAGC-3
TNF-α	5-AAATGGGCTCCCTCTCATCAGTTC-3	5-TCTGCTTGGTGGTTTGCTACGAC-3