Olive Leaves Extract and Oleuropein Improve Insulin Sensitivity in 3T3-L1 Cells and in High-Fat Diet-Treated Rats via PI3K/AkT Signaling Pathway

Table 3

Scoring criteria of immunohistochemistry assay with antibodies including in signaling pathway in WAT tissue.

p-AKT
Staining positive cells			Staining intensity		Final score product
Groups	Percent%	Score 1	Intensity	Score 2	Score 1 X Score 2	Score 3
CTR	51%-75%	3	Strong	3	9-12	3+(+++)
HFD	6%-25%	1	Weak	1	2-4	1+(+)
HFD + OLA	26%-50%	2	Moderate	2	5-8	2+(++)
p-IRS
CTR	51%-75%	3	Strong	3	9-12	3+(+++)
HFD	6%-25%	1	Weak	1	2-4	1+(+)
HFD + OLA	26%-50%	2	Moderate	2	5-8	2+(++)
GLUT-4
CTR	51%-75%	3	Strong	3	9-12	3+(+++)
HFD	<5%	0	Absent	0	0-1	0(-)
HFD + OLA	6%-25%	1	Weak	1	2-4	1+(+)

Note: scoring results are determined by screening 12 consecutive microscopic fields. Percent positive cells (score 1) multiply staining intensity (score 2) equals to final product score (score 3). Either 0 or (-) depicts negative staining. An individual slide had <5% of staining cells (= 0) with a staining intensity of 1 (= weak) which would generate a final product score of ; another slide had 80% of staining cells (= 4) with a staining intensity of 3 (= strong) which would give a final product score of .